Codon bias in E. Coli Essay Example for Free

Codon bias in E. Coli Essay The nature of the gene codon varies among organisms. Codon preferences have been considered on the perspectives of translational efficiency and fidelity and the selective and non selective biases operating during DNA transcription replication and repair processes. Variations in tRNA on codon bias of highly expressed genes during rapid growth phase of E. coli exist. Codon selectivity is influenced by codon/anticodon interaction strength, site specific codon biases, time of replication, codon context, or evolutional age. Codon preferences among gene classes in E. coli are grouped according to the following comparisons; correlation of codon biases with level of gene expression, organisation of genome according to genome partitions based on size, codons use for genes characterized by function and cellular localization of gene products, gene size, comparisons that divide all genes by similarity of codon usage or amino acid usage or similarity of a reduced set of amino acid or codons and characterization of alien genes. The Codon Adaptation Index (CIA) is a qualitative measure for assessing codon bias. Ribosomal protein (RP) genes and membrane genes are genes that are highly expressed during fast growth and CIA and codon bias (CB) values are inversely correlated with respect to RP genes. Most ribosomal proteins are highly expressed during the E. coli exponential growth phase during which most genes facilitating translation are highly expressed. Codon preferences differ among highly expressed genes relative to the average gene for certain amino acid types, especially disparities for alanine, aspartine, histidine, isoleucine, phenealanine, threonine and valine. There is a high correlation between optimal codons and level of gene expression. In relation to E. coli genes, codon biases generally increase with increasing protein molecular weight abundance. Codon usage differences generally decrease with respect to protein molecular abundance when compared to RP. Negative correlation in the degree of protein molar abundance relative to tRN genes or other sub classes of the translation functional category is not evident. The molar abundance and codon usage differences unequivocally correlate negatively with the RP gene family and positively with the average E. coli gene respectively. The E. coli genome is homogeneous with relatively weak codon biases among the genes distributed over the genome. Codon bias does not depend on timing in the replication cycle except near the ter region. The deviation in codon usage from RP and tRN genes is most emphatic at ter region. Relative codon usage bias among 5’ middle and 3’ parts of genes in E.coli show that the middle and the last third of genes are more similar in codon usage than either is to the initial third of the gene. There also exists a difference in the frequencies of 3 G+C near the oriC than near the ter region. Different bacterial genomes display variation in their overall G and C content, attributed to varying mutational mechanisms and processes. For eukaryotic genomes site 3 G+C frequencies decrease with increasing gene length. Alien genes are characterized in terms of extreme codon bias relative to average E.coli genes and high relative to RPs. These genes are of unknown function and are either GC rich or AT rich. Extremes in codon bias are for identifying pathogenicity islands and developing gene classes reflecting difference expression levels in untypical events. When the genome is divided into contigs, gene classes and dicodon bias is most pronounced between the gene classes of the region about the oriC versus the ter region. Dicodon bias increases with gene size and compared to the average gene dicodon biases are constant throughout the genome. When genes are divided into thirds, the dicodon biases of the 5’ third, middle third . 3’ third parts of genes are similar though level of bias is about twice that of straight codon bias. Codon and dicodon bias correlations for E. coli genes were evaluated for level of expression, contrast along genes, size of contigs around genome and gene size classes. Explanations for codon bias have involved combinations of selection and mutational pressures. The RPs and amminoacyl tRNA synthetases are highly expressed gene classes during exponential growth of E.coli. Codon biases for RP genes are much more extreme than for tRN genes. The greater the abundance a gene product, the more its codon usage resembles hat of RPs, but this is not the case for comparisons to tRN genes or protein genes essential to translational activities. For gene classes RP and tRN, the source of codon bias differs significantly. RP are among the most deviant from the average E. coli gene. It is suggested that codon usage and tRNA abundance are correlated for highly expressed genes to match substrate levels with cellular demands. Hence RP genes which are small single domain proteins show high codon usage correlation with overall E. coli codon usage and tRNA do not show this bias although they are highly expressed. The middle and final 3? end of genes entail the same levels of codon biases. The rare codon hypothesis for domains and secondary structures argues that the use of repetitive rare codons might reduce translational rate and induce translation pauses allowing protein domains and suitable secondary structures to fold into native structural conformation. There are differences in prokaryotic and eukaryotic translational mechanisms. These differences may be important in translation initiation or early stages of translation. Highly biased slowly translated codon pairs are more closely correlated with levels of expression than with protein length. Understanding basis of codon usage is of interest with respect to fundamental evolutionary questions, gene prediction, gene classification and design of optimal expression vectors. Codon usage programs are essential for gene finding and analysis of prokaryotic and eukaryotic genomes.

Carrying out Administrative Skills Based on a Project on the Old Bailey :: Administrative Operations Old Bailey Essays

Carrying out Administrative Skills Based on a Project on the Old Bailey In this coursework, I have to show I am capable of showing a variety of administrative skills for a project of my own choice. I have chosen to base my project on the Old Bailey. In order to carry out my administrative skills, I had to carry out an activity such as, setting up and participating in an away day trip to an organisation for at least six people, in which I am the key administrator. I also have to show knowledge and understanding of the roles of administrators in organisations. In this coursework I’ am required to work in a group of people that have specific roles in the group and plan and organize (or administer) our own event for AVCE Business Studies Year 2, unit 20 – Admin Ops. Our event has to be well planned and multi-layered, as this will allow each individual in the group to act out their role. My group is built up of 6 people. They are, Rachel, Chris, Sophie, Asif, Ashraf and me. We have all worked together in Unit 1 (Business At Work) and have all obtained experience of producing information. Examples are in, Unit 3, 4 and 10 (Marketing, HR and Customer Service). We all set each other positions or roles in the group in order to get our event started up. Below are my group’s roles/positions. * Rachel – Boss * Sophie – PR/Phones * Chris – Planner * Asif – IT * Ashraf – Research * Ravi – Research and minute taker. As you can see from above I have a second job role. This is taking down minutes (or notes) at every lesson. This is to make sure we don’t forget to mention anything in-group discussions and can help all of us in every meeting the group holds. These are all our roles in the group. We are also called Group A as there are 2 groups that have to organise an event. We also have to organise and record our event by taking a camera. IT is a critical path as it allows us to broaden our knowledge on the place we want to go to for our event. We also must use a computer database to store information and manage our project efficiently to ensure a smooth running of it. We had our first meeting at 11.33am on the 16/9/2004 and in the meeting we had to be sorted n groups and then allocate roles to each other. We also had an agenda. The agenda was to discuss project

Individualism vs Collectivism

Individualism and Collectivism Culture Abstract This essay discusses different aspects of Individualism and collectivism culture. Although every culture is different, Individualist and collectivism have a different implication for social organization. In this essay I able use my personal experience as a foreigner to help the readers understand the subject. In addition to the research I have done I was able to find information to help me fully understand how the two concepts impacted different countries such as the U. S.A, China and Burkina Faso. There are about a hundred and nighty-six countries on this planet, each one of them has a variety of cultures and traditions. Cultures are typically divided into two categories: collectivist and individualist. Individualist cultures, such as those of the United Sates and Western Europe, value personal achievement resulting in a strong sense of competition. Collectivist cultures, such as those of China, Korea, and Japan, emphasize family and w ork group goals above individual needs or desires.From a personal preference I would prefer a collectivist society but orders of social behavior change from culture to culture. I will now explain my experience coming in the United States to study. Being an international student from Burkina Faso, a country that has more than sixty ethnic groups with all different languages and dialects. I myself speak three languages, Moore my first and French second. The Western cultures are more individualist than collectivist Eastern cultures. The United States is considered to be an individualist country, so when I first got here, the transfer was very hard.I met American friends at U. G. A, despite been very nice it was hard sometimes to understand each other, as our cultures are different. The terms individualism and collectivism are usually used to express different cultures. An individualistic culture is where individuals are said to be bounded, autonomous, distinct from others, and emphasiz ing one’s own goals and agency rather than those of others (Mascolo M. F. & Li J. , 2004, p. 27). As it was stated in the first paragraph the United States is listed as having an individualist society.Some people would offer themselves, their family and friend as an anecdotal counter example to American individualism, but â€Å"both individualist and collectivist orientations may coexist within individuals cultures (Kim U. & Trandis H. C. & Choi S. C. , 1994, p. 123). For example; my best friend in Burkina Faso, born and raised there grew up to become an individualist. She say it herself, â€Å"I do not like to share†. A person may live in a traditionally collectivistic culture and may still express individualistic behaviors within that culture, just like my friend.The culture in Burkina is known for being hostile and as for myself being born there, and having a total nine siblings, we all shared everything, such as cloth, food, toys and many other things. So growing in this environment I have learned to share not only with brother and sisters but sometimes cousins. The traditional views of individualism and collectivism suggest that Eastern countries will exhibit collectivistic tendencies while Western countries will exhibit individualistic tendencies (Parker R. S. , Haytko D. L. , & Hermans C. M. , 2009). According Sen L. H. (2004).Being raised in a collectivist environment, the transition at UGA was a bit complicated. Many of my friends at UGA were born and raised in Georgia; some of them have a different way of approaching this subject. For example one of them had a total different situation than mine. He has 2 sisters and was told by his parents that if they each get something each of them have the right not to share. Since both individualist and collectivist orientations may coexist within the two cultures, it is practically impossible to say that everyone in western countries is an individualist or that everyone in eastern countries and A frica is a collectivist.As a within the communities it might be possible. For example most of the time in my country if someone is getting married everyone is pretty much invited. One will have thousands of people at their wedding to show support to the newlyweds. Studies suggest the well educated are more individualistic than the less educated. City dwellers are more individualistic than rural residents. Men are more individualistic than women, and young more than old. Thus, we need to consider peoples' experiences when interacting with them (Kanchier C. , 2007).The same individualism that has led to disaster, such as the mortgage crisis, because it was pursued to excess will also be the source of America's eventual resurgence. At its best, American individualism embraces the notion that every person matters. Increasingly, in American cities especially on the coasts, you will find that social categories are less important than individual histories. The U. S media will rather gossip about bill Clinton affair and Paris Hilton’s sex tapes than talk about important subjects that actually affects people’s life like the increasing dangerousness of tuberculosis as it becomes more immune to anti biotic.By contrast, collectivist are connected and interdependent, stressing roles and relationship, more than individual uniqueness; they are principally motivated to pure group goals rather than their own (Mascolo M. F. & Li J. , 2004, p. 27). Like individualism, within every culture there will be both individualist and collectivist people. Now lets take a look at the aspect of collectivism in China. Chinese culture is indeed more collectivist than individualist, and individualist values such as self-interest tend not to be present. (Kim U. & Trandis H.C. & Choi S. C. , 1994, p. 154). Hui in 1988 developed the individualism-collectivism scale (INDOL scale); it comprises six subscales purporting to measure a person’s collectivism orientation toward targe t groups (Kim U. & Trandis H. C. & Choi S. C. , 1994, p. 149). In his research Hui compared the scores of Chinese university students to those of American university students. Taken together, these results indicate that Chinese culture may be characterized as predominantly collectivist as well as anti-individualist. (Kim U. & Trandis H.C. & Choi S. C. , 1994, p. 154) For example the Chinese culture divorce is considered be dishonorable they believe that the marriage of two people is the union of two families. According to Luke Metcalfe, 2013, the divorce rate in china is 0. 79 per 1000people. As compared to the U. S. it is very common and some areas considered the norm. Luke Metcalfe, 2013 stated that The U. S divorce rate is at 4. 95 per 1000 people, which is the highest country with divorce rate. Individualist and collectivism have a different implication for social organization.The components of social organization presented in the book â€Å"Individualism and Collectivism† are very important; they are combined into more inclusive major components, of which there are five: values, autonomy/conformity, responsibility, achievement and self-reliance/interdependence. As an example of autonomy/conformity, an my best friend from Burkina Faso would want the right to privacy she believe that everyone should mind their own business. Their privacy should be respected. While on the other side I would believe that one’s business is also the business of the group; friends should be concerned with each other’s personal matters.Individualism and Collectivism are both needed in a society, inside every cultures will exists both concepts. I was raised in a collectivist environment but could decide to become an individualist; it may be an unconscious choice, but it happens very frequently. The two concepts have their advantages and disadvantages. When I was a kid I wanted to learn how to rollerblade because my friends knew how to. I was too proud to ask f or their help, so I have decided to teach myself how to uses the rollerblades. I certainly did, but at what cost? Multiple injuries and it took forever before I could really get the hint of it.I now realize that it would have been less painful if I just asked my friends help. Because I believe that collective work can make one attain greater success. Because together we are stronger. It is important for us readers to remember that individualism and collectivism each serve their own purposes, they are part of the reasons why our society is the way it is today. References Kanchier, C. (2007, May 12). Are you part of collective? ; we are not all individualism. How you view your-self influences how you relate to others at work. The Gazette, p. . Kim U. & Trandis H. C. & Choi S. C. (1994). Individualism and collectivism. United States of America: SAGE. Mascolo M. F. & Li J. (2004). Culture and development selves: beyond dichotomization. San Francisco: Wiley company. NationMaste. (2003-20 13). Luke Metcalfe. Retrieved from: http://www. nationmaster. com/graph/peo_div_rat-people-divorce-rate Parker R. S. , Haytko D. L. , & Hermans C. M. , (2009). Individualism and Collectivism: Reconsidering old Assumptions. Sen, L. H. (2004, May 7). Society needs individualism. The Straits Times, p.

Pros and Cons of Government Intervention Essay - 894 Words

Objective This specific report addresses the pros and cons of how much government intervention in the marketplace is necessary and appropriate. This report will cover four authors, Cunningham, Green, Friedman and Pertschuk. The authors have written extensively on consumerism and the protection thereof. This author’s goal is to break down some of the theories, which authors seem akin in their beliefs and which authors’ views are bipolar opposites. Green states there be only should be two types of government regulations 1) economic regulations and 2) health and safety regulations. He states the only important pieces of consumer legislation to emerge from Congress in the past three years were the â€Å"Consumer Protection Safety†¦show more content†¦Friedman views the consumer as strong and is totally interested in efficiency. He does note however the FDA had done a good job of keeping us from harmful medication. The Cunningham’s objective of their resear ch was to determine how many individuals in various sectors of society know about the consumer protection laws and their rights as consumers. These were included, but not limited to false advertising, false retail advertising, credit regulations, credit cards, labeling, truth in lending and deceptive retail practices. These authors view the consumer relatively weak due to a lack of knowledge. They also feel the low incomes are no match for the sophisticated marketer. There these authors are interested in equity. The most important implication derives from the lack of information demonstrated by all income segments of the consumer sample. This situation suggests that what is needed is not more and tougher laws, but rather more information made available to ore individuals concerning their rights as consumers. The present research has demonstrated that one other variable is important: with the exception of a few areas of the law, both consumers and attorneys know very little abo ut their rights as consumers. This was particularly true in areas such as door-door selling, false or deceptive advertising, false or deceptive retail practices. More and better information concerning consumers’ rights must be provided to individuals of allShow MoreRelated Government Intervention in the Free Market Essay1125 Words   |  5 Pages It is not only unnecessary for the government to intervene to maintain a free market, it is extremely wrong. Intervention by any outside party in corporate matters is inappropriate and basically contradicts the meaning of a free market. There are some positive effects government intervention could produce. These pros are, in fact, few, and questionable, at that. Take for instance, the situation with Microsoft. The government is sticking its nose in where it doesnt belong. 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